Voltage-controlled enzyme-catalyzed glucose-gluconolactone conversion using a field-effect enzymatic detector.

Voltage-Controlled Enzyme-Catalyzed Glucoseâ•fiGluconolactone Conversion Using A Field-Effect Enzymatic Detector

Reactor design for minimizing product inhibition during enzymatic lignocellulose hydrolysis: II. Quantification of inhibition and suitability of membrane reactors.

Product inhibition of cellulolytic enzymes affects the efficiency of the biocatalytic conversion of lignocellulosic biomass to ethanol and other valuable products. New strategies that focus on reactor designs encompassing product removal, notably glucose removal, during enzymatic cellulose conversion are required for alleviation of glucose product inhibition. Supported by numerous calculations ...

Ultrasensitive biosensing on the zepto-molar level.

Detection of analytes on the zepto-molar (10(-21) M) level has been achieved using a field-effect bio-detector. By applying a gating voltage to enzymes immobilized on the working electrode of the detector, amplification of the biocatalytic current was observed. The amplification is attributed to the modification of the tunnel barrier between the enzyme and the electrode by the gating voltage-in...

Novel Pathway for Alcoholic Fermentation of -Gluconolactone in the Yeast Saccharomyces bulderi

Under anaerobic conditions, the yeast Saccharomyces bulderi rapidly ferments -gluconolactone to ethanol and carbon dioxide. We propose that a novel pathway for -gluconolactone fermentation operates in this yeast. In this pathway, -gluconolactone is first reduced to glucose via an NADPH-dependent glucose dehydrogenase (EC 1.1.1.47). After phosphorylation, half of the glucose is metabolized via t...

The effect of AMP on inhibition of muscle phosphorylase a by glucose derivatives.

Kinetic studies of the inhibition of rabbit muscle phosphorylase a D-glucan:orthophosphate alpha-D-glucosyltransferase (EC 2.4.1.1) by various glucose derivatives indicate that glucose derivatives in the chair conformation (glucose, 2-deoxyglucose, glucosyl fluoride, and 1-O-methylglucose) stabilize the "T" form of phosphorylase a, whereas those in the half-chair conformation (1,5-gluconolacton...